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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
31/01/2019 |
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Data da última atualização: |
14/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORATI, L. S.; TAGGART, J. B.; VARELA, E. S.; ARARIPE, J.; WEHNER, S.; MIGAUD, H. |
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Afiliação: |
LUCAS SIMON TORATI, CNPASA; JOHN BERNARD TAGGART, UNIVERSITY OF STIRLING, Scotland-UK; EDUARDO SOUSA VARELA, CNPASA; JULIANA ARARIPE, UNIVERSIDADE FEDERAL DO PARÁ, Bragança-PA; STEFANIE WEHNER, MAX PLANCK INSTITUTE, Munich; HERVÉ MIGAUD, UNIVERSITY OF STIRLING, Scotland-UK. |
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Título: |
Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
BMC Genetics, v. 20, n. 13, Jan. 2019. |
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ISSN: |
1471-2156 |
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DOI: |
10.1186/s12863-018-0711-y |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region. MenosBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a d... Mostrar Tudo |
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Palavras-Chave: |
Araguaia; Tocantins. |
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Thesagro: |
Arapauma Gigas; Conservação; Genética Animal; Pirarucu. |
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Thesaurus Nal: |
Amazonia; Aquaculture; Arapaima gigas; Fisheries; Rivers; Species diversity. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191822/1/CNPASA-2019-bmcgenetics.pdf
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Marc: |
LEADER 03345naa a2200349 a 4500
001 2105220
005 2020-02-14
008 2019 bl uuuu u00u1 u #d
022 $a1471-2156
024 7 $a10.1186/s12863-018-0711-y$2DOI
100 1 $aTORATI, L. S.
245 $aGenetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins.$h[electronic resource]
260 $c2019
520 $aBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region.
650 $aAmazonia
650 $aAquaculture
650 $aArapaima gigas
650 $aFisheries
650 $aRivers
650 $aSpecies diversity
650 $aArapauma Gigas
650 $aConservação
650 $aGenética Animal
650 $aPirarucu
653 $aAraguaia
653 $aTocantins
700 1 $aTAGGART, J. B.
700 1 $aVARELA, E. S.
700 1 $aARARIPE, J.
700 1 $aWEHNER, S.
700 1 $aMIGAUD, H.
773 $tBMC Genetics$gv. 20, n. 13, Jan. 2019.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/11/2017 |
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Data da última atualização: |
03/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SOUZA, I. R. P. de; MACEDO, G. A. R.; BARBOSA, M. H. P.; BARROS, B. de A.; CARVALHO, S. G. M.; XAVIER, A. da S.; GONÇALVES, I. A. M. |
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Afiliação: |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; Geraldo Antônio Resende Macêdo, Epamig; Márcio Henrique Pereira Barbosa, Universidade Federal de Viçosa; BEATRIZ DE ALMEIDA BARROS, CNPMS; Samanta Gabriela Medeiros Carvalho, UFLA; André da Silva Xavier, Bolsista; Isabella Aparecida Maia Gonçalves, UNIFEMM. |
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Título: |
Reaction of sugarcane genotypes to strains of the sugarcane mosaic virus. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
International Journal of Current Research, v. 9, n. 10, p. 59112-59119, Oct. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane crops are cultivated in nearly all tropical and sub-tropical regions worldwide andhave a prominent position in the agricultural scenario in Brazil. However, viral diseases can threaten the production of this important commodity leading to large production losses. In the present study, we evaluatedthe reaction of 20 sugarcane genotypes independently inoculated with two different strains of Sugarcane mosaic virus (SCMV) isolated from naturally infected sugarcane (SCMV-SGC) and maize (SCMV-MZ). The maize inbred line L19 was used as a control of susceptibility to the SCMV-MZ strain. Symptoms intensity was evaluated through a visual scale with three levels of severity: weak, intermediate, and intense. The viral infection was confirmed by PCR and DNA sequencing. We observed that, although both strains were able to infect sugarcane genotypes and the maize inbred line, SCMV-SGC was more aggressive, resulting in only four resistant genotypes: IN84-58 (S. spontaneum), RB855536, RB 928064, and SP71-6163. Thirteen genotypes were resistant to SCMV-MZ: IN84-58 (S.spontaneum), NA56-79, CB47-355,CB49-260, RB72454, RB855113, RB855536, RB867515, RB928064, SP70-1143, SP71-1406, SP71-6163, and SP81-3250. This is the first report showingSCMV strains capable of cross-infectingand causing mosaic in sugarcane and maize. Our data emphasize the importance of continuous monitoring and screening for virus resistantgenotypes to be used in breeding programs for the development of new resistant cultivars. MenosSugarcane crops are cultivated in nearly all tropical and sub-tropical regions worldwide andhave a prominent position in the agricultural scenario in Brazil. However, viral diseases can threaten the production of this important commodity leading to large production losses. In the present study, we evaluatedthe reaction of 20 sugarcane genotypes independently inoculated with two different strains of Sugarcane mosaic virus (SCMV) isolated from naturally infected sugarcane (SCMV-SGC) and maize (SCMV-MZ). The maize inbred line L19 was used as a control of susceptibility to the SCMV-MZ strain. Symptoms intensity was evaluated through a visual scale with three levels of severity: weak, intermediate, and intense. The viral infection was confirmed by PCR and DNA sequencing. We observed that, although both strains were able to infect sugarcane genotypes and the maize inbred line, SCMV-SGC was more aggressive, resulting in only four resistant genotypes: IN84-58 (S. spontaneum), RB855536, RB 928064, and SP71-6163. Thirteen genotypes were resistant to SCMV-MZ: IN84-58 (S.spontaneum), NA56-79, CB47-355,CB49-260, RB72454, RB855113, RB855536, RB867515, RB928064, SP70-1143, SP71-1406, SP71-6163, and SP81-3250. This is the first report showingSCMV strains capable of cross-infectingand causing mosaic in sugarcane and maize. Our data emphasize the importance of continuous monitoring and screening for virus resistantgenotypes to be used in breeding programs for the development of new resistant ... Mostrar Tudo |
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Thesagro: |
Cana de açucar; Doença de planta; Mosaico. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166067/1/Reaction-sugarcane.pdf
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Marc: |
LEADER 02211naa a2200229 a 4500
001 2078844
005 2017-11-03
008 2017 bl uuuu u00u1 u #d
100 1 $aSOUZA, I. R. P. de
245 $aReaction of sugarcane genotypes to strains of the sugarcane mosaic virus.$h[electronic resource]
260 $c2017
520 $aSugarcane crops are cultivated in nearly all tropical and sub-tropical regions worldwide andhave a prominent position in the agricultural scenario in Brazil. However, viral diseases can threaten the production of this important commodity leading to large production losses. In the present study, we evaluatedthe reaction of 20 sugarcane genotypes independently inoculated with two different strains of Sugarcane mosaic virus (SCMV) isolated from naturally infected sugarcane (SCMV-SGC) and maize (SCMV-MZ). The maize inbred line L19 was used as a control of susceptibility to the SCMV-MZ strain. Symptoms intensity was evaluated through a visual scale with three levels of severity: weak, intermediate, and intense. The viral infection was confirmed by PCR and DNA sequencing. We observed that, although both strains were able to infect sugarcane genotypes and the maize inbred line, SCMV-SGC was more aggressive, resulting in only four resistant genotypes: IN84-58 (S. spontaneum), RB855536, RB 928064, and SP71-6163. Thirteen genotypes were resistant to SCMV-MZ: IN84-58 (S.spontaneum), NA56-79, CB47-355,CB49-260, RB72454, RB855113, RB855536, RB867515, RB928064, SP70-1143, SP71-1406, SP71-6163, and SP81-3250. This is the first report showingSCMV strains capable of cross-infectingand causing mosaic in sugarcane and maize. Our data emphasize the importance of continuous monitoring and screening for virus resistantgenotypes to be used in breeding programs for the development of new resistant cultivars.
650 $aCana de açucar
650 $aDoença de planta
650 $aMosaico
700 1 $aMACEDO, G. A. R.
700 1 $aBARBOSA, M. H. P.
700 1 $aBARROS, B. de A.
700 1 $aCARVALHO, S. G. M.
700 1 $aXAVIER, A. da S.
700 1 $aGONÇALVES, I. A. M.
773 $tInternational Journal of Current Research$gv. 9, n. 10, p. 59112-59119, Oct. 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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